Understanding the reproductive ecology of endangered freshwater species is critical for effective conservation. This study investigates the spawning-related movements and nesting behaviour of adult trout cod (Maccullochella macquariensis) in Bendora Reservoir, Australian Capital Territory. Using acoustic telemetry, we monitored the movements of tagged adult individuals across multiple years to determine if spatial use differed during breeding (September – November inclusive). To locate nesting areas, we conducted a boat-based visual survey of the perimeter of the reservoir, as well as snorkelling surveys of subset of rocky outcrops and banks. Active nests were monitored for adult guarding behaviour using both drone video and underwater video. Telemetry data indicated increased activity during the spawning period, with several individuals repeatedly traversing the length of the reservoir. In total, nine active nests were located around the perimeter of the reservoir. Nests were always on rock or concrete, and were in depths from 0.8 – 4 m. All but one nest was found within 3 m of another nest. In one instance, a cluster of five nests was located within a 10 m length of shoreline. Guarding behaviour by adult trout cod was confirmed on eight of nine nests, with one nest never having an adult observed on or near it. The spatial distribution of nests suggests specific habitat preferences, with trout cod favouring rock / concrete shelves for nesting sites. Our findings provide valuable insights into the breeding ecology of trout cod in lentic systems, emphasizing the need for targeted habitat protection. Our study increases the understanding of movement patterns and nesting preferences of trout cod, which can better inform management strategies aimed at conserving this endangered species both in lentic and lotic habitats.