Over a third of shark species globally are threatened with extinction, prompting growing public concern. Recently, 97 shark species were added to the CITES Appendix 2, emphasising the relevance and urgency of the issue. Historically traded for their fins, the demand and trade for shark meat has been rising, with meat sold globally under a variety of different ambiguous labels. In Australia, shark meat is typically sold under the label flake, however, this is non-mandatory label that is recommended to be either gummy shark or New Zealand rig shark. Here, we identified the shark species sold in the flake trade across the supply chain, encompassing fishers, markets, and retailers, finding a variety of threatened and CITES-listed species being sold as flake. At the same time, we aimed to develop a more cost-effective approach for screening samples and rapid diagnostics. We designed species-specific DNA amplification assays for school shark and gummy shark, using loop-mediated isothermal amplification (LAMP) to boost support for field-based applications. Overall, these assays can enable faster, more cost-effective, presumptive identifications of target species, facilitating broader sampling and expanded monitoring applications.